Abstract:This study aimed to prepare a Polygonati Rhizoma and black bone chicken soup,and investigate its immunomodulatory effect and mechanism through integrated sensory evaluation,network pharmacology,and animal experiment.First,potential targets associated with the immunomodulatory effects of the soup were screened from the database,followed by enrichment analysis.Then,the soup was prepared and subjected to sensory evaluation.Finally,an immunosuppressed mouse model induced by cyclophosphamide（CTX）received different doses of freeze-dried powder solution of the soup by gavage to assess its effect on immune function.According to the results,a total of 206 potential targets were identified to be related to the immune-enhancing effect of the soup,which were enriched in signaling pathways such as the chemokine,T-cell receptor,and phosphatidylinositol 3-kinase（PI3K-Akt）pathways.Sensory evaluation indicated that the prepared soup was transparent and light amber,characterized by robust aromatic properties,dominant umami flavor,and a distinctive sweet aftertaste.In the animal experiments,compared to the control group,the CTX group exhibited a significantly decreased relative body weight,along with decreased spleen and thymus index,serum immunoglobulin（IgA,IgG,and IgM）levels,and hematological parameters [white blood cells（WBC）,red blood cells（RBC）,hemoglobin（HGB）,and lymphocytes（LYMPH）]（P<0.05,P<0.01）.In contrast,intervention with different doses of the soup alleviated the decrease in relative body weight to varying degrees and significantly increased the aforementioned immune organ index,immunoglobulin levels,and hematological markers.