Abstract:According to the principle of specific binding of chemical groups to antibodies，molecular biological methods and immunological methods were employed to study the polymerase chain reaction（PCR）amplification products of Vibrio parahaemolyticus through binding of the amplification products of microfluidic chip PCR with colloidal gold antibodies.PCR amplification was performed for V.parahaemolyticus，9 strains closely related to V.parahaemolyticus，and 6 food-borne pathogen strains to evaluate the specificity of the method.The fresh bacterial suspension was subjected to 10-fold gradient dilution，and then PCR amplification was carried out to test the sensitivity of the PCR amplification products of V. parahaemolyticus. The shrimp samples were polluted by the bacterial suspension and then used to verify the sensitivity and reliability of this method in mixed samples. The results showed that the nucleic acid capture colloidal gold strips could specifically detect the PCR amplification products of V. parahaemolyticus，and there was no cross-reaction with other strains. The sensitivity of the microfluidic chip PCR-colloidal gold test strips in the detection of V.parahaemolyticus suspension could reach 5 CFU/mL，consistent with agarose gel electrophoresis results.This method enabled rapid，sensitive，and effective detection of V.parahaemolyticus.