Abstract:Lentilactobacillus parabuchneri C41 is a riboflavin-producing strain isolated from tomato fermentation broth.This study employed whole genome sequencing and analysis to explore the mechanism of riboflavin production and the potential hazards of the C41 strain.The whole genome sequence of the C41 strain was obtained by Pacbio and Illumina sequencing.The functional mechanism and potential risks of the strain were analyzed by coding gene prediction and annotation.The results showed that the genome of strain C41 contained circular DNA and three plasmids.The size of the circular DNA was 2 650 018 bp,and the GC content was 43.53%.The sizes of plasmid A,plasmid B,and plasmid C were 58 659 bp,50 453 bp,and 49 016 bp,respectively.2 802 coding genes,64 tRNA genes,and 15 rRNA genes were predicted.Through KEGG annotation,it was discovered that the chromosome of strain C41 contained the enzyme-encoding genes required for the de novo synthesis of riboflavin.Furthermore,the analysis of carbon-hydrocarbon active enzymes and secondary metabolite synthesis genes jointly contained 66 carbon-hydrocarbon active enzyme coding genes,one type III polyketide synthase gene cluster,and two ribosomally synthesized and post-translationally modified peptide gene clusters.The comparison analysis between the pathogenicity factor database of pathogenic bacteria and the comprehensive antibiotic resistance database revealed that no genes in the C41 genome were annotated as virulence genes,while one gene was annotated as a gene related to antibiotic resistance.The results demonstrated that the C41 strain possessed the capacity for the de novo synthesis of riboflavin and exhibited favorable safety profiles,rendering it promising for further research.