Abstract:A comparative analysis was conducted on the nutritional and medicinal components of buffalo tripe juice obtained through traditional and improved extraction methods,along with an investigation into its inhibitory activity against α-glucosidase.The improved extraction method employed a high-speed rotary pulverizer operating at 18 000 r/min to grind the grass in the tripe into pulp,followed by filtration and juice extraction.The contents of vitamins,proteins,and total amino acids in the juice obtained using the improved method were all higher than those in the traditional method,measuring 163 μg/mL,570 mg/100 g,and 270.127 μg/mL,respectively.The contents of the medicinal components were also enhanced.Specifically,the xylose content was 8.40 μg/mL,the saponin content was as high as 238.21 mg/g,and 12 types of functional flavonoids were identified.Among them,the contents of quercetin,baicalein,and luteolin C,C-dipentoside reached 13.624,18.427,and 16.293 mg/g,respectively.Notably,quercetin,baicalein,and other components exhibited blood glucoselowering effects.Both extraction methods produced buffalo tripe juice that exhibited significant inhibitory effects on α-glucosidase activity,with the inhibition rate being proportional to the concentration of the juice.The initial concentration of the crude juice from the improved method achieved an inhibition rate of up to 82%,with an half maximal inhibitory concentration（IC50） value of 0.29 mg GAE/mL against α-glucosidase,demonstrating a pronounced hypoglycemic effect.