Abstract:A method for determining 46 insecticide residues in animal-derived food by pass-through solidphase extraction-ultra high performance liquid chromatography-tandem mass spectrometry was established.The animal-derived food samples were dispersed with water， extracted with acetonitrile， and purified with HMR-Lipid solid-phase extraction column. The analytes were separated on a XSelect HSS T3 column by gradient elution with methanol and water as the mobile phases. Then， quantification of the analytes was performed by the matrix-matched standard curve external standard method. The results showed that the 46 insecticides had good linear relationships within their respective ranges， with the correlation coefficients greater than 0.995. The limits of quantification （LOQ） of the method for the insecticides were 1.0-10.0 μg/kg. With pork，pig kidney， egg， and milk as matrixes， the recovery rates at three spiking levels （LOQ， 10 LOQ， and 50 LOQ） ranged from 65% to 110%， with relative standard deviations of 1.9% to 10.6%. The established method was efficient， simple， and accurate， thus being suitable for rapid quantitation of 46 insecticide residues in animal-derived food products such as livestock and poultry meat， offal， eggs， and raw milk.