食品研究与开发:2025,46(24):1180-186
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铜藻岩藻多糖离子色谱指纹图谱构建
蔡树芸1,2,陈晖1,3,4,施丽君1,杨婷1,黄晓梅3,陈伟珠1,3,4,张怡评1,2,3,4*
(1.自然资源部第三海洋研究所 海洋生物资源开发利用工程技术创新中心,福建 厦门 361005;2.福建中医药大学 药学院,福建 福州 350122;3.厦门海洋职业技术学院 海洋生物学院,福建 厦门 361100;4.福建省海岛资源生态监测与保护利用重点实验室,福建 福州 350400)
Establishment of the Ion Chromatography Fingerprint of Fucoidan from Sargassum horneri
CAI Shuyun1,2,CHEN Hui1,3,4,SHI Lijun1,YANG Ting1,HUANG Xiaomei3,CHEN Weizhu1,3,4,ZHANG Yiping1,2,3,4*
(1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China;2.College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China;3.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,Fujian,China;4.Fujian Key Laboratory of Island Monitoring and Ecological Development,Fuzhou 350400,Fujian,China)
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投稿时间:2024-08-09    
中文摘要: 为建立离子色谱-脉冲安培检测法测定铜藻岩藻多糖中单糖组成的方法,该文采用酸水解方法将岩藻多糖水解成单糖,采用离子色谱(ion chromatography,IC)法对其单糖组成进行测定,构建岩藻多糖指纹图谱,并进行相似度分析。通过建立10个批次铜藻岩藻多糖样品指纹图谱,相似度高于0.97,确定6个共有色谱峰,并通过标准品比对分析,确认铜藻岩藻多糖主要由岩藻糖、半乳糖和木糖等单糖组成。同时与其他多糖的图谱进行对比分析,该方法可以用于区分不同来源的海藻多糖。
Abstract:A method for determining the monosaccharide composition of fucoidan from Sargassum horneri was established based on ion chromatography-pulsed amperometric detection.Acid was used to hydrolyze fucoidan into monosaccharides,and ion chromatography was employed to determine the monosaccharide composition.Fucoidan fingerprints were established and their similarity was analyzed.The fingerprints of ten batches of fucoidan samples from S.horneri had the similarity higher than 0.97,with six common chromatographic peaks identified.Through standard sample comparison,it was identified that fucoidan from S.horneri mainly consisted of monosaccharides such as fucose,galactose,and xylose.By comparing and analyzing the chromatograms with those of other polysaccharides, this method can be used to distinguish seaweed polysaccharides from different sources.
文章编号:202524023     中图分类号:    文献标志码:
基金项目:厦门市海洋与渔业发展专项资金项目(23CZP009HJ11);舟山市科技计划项目(2023C13017、2023C13014);福建省科技计划项目(2021N0029);厦门市科技计划项目(3502Z20221036)
Author NameAffiliation
CAI Shuyun 1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China2.College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China 
CHEN Hui 1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China3.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,Fujian,China4.Fujian Key Laboratory of Island Monitoring and Ecological Development,Fuzhou 350400,Fujian,China 
SHI Lijun 1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China 
YANG Ting 1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China 
HUANG Xiaomei 3.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,Fujian,China 
CHEN Weizhu 1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China3.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,Fujian,China4.Fujian Key Laboratory of Island Monitoring and Ecological Development,Fuzhou 350400,Fujian,China 
ZHANG Yiping 1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China2.College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China3.School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,Fujian,China4.Fujian Key Laboratory of Island Monitoring and Ecological Development,Fuzhou 350400,Fujian,China 
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