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投稿时间:2024-09-27
投稿时间:2024-09-27
中文摘要: 为确定恰玛古多糖生产过程中原料的最优干燥方式,以不同产地恰玛古为原料,通过水提醇沉法制备多糖。采用紫外吸收光谱法、傅里叶红外光谱法、刚果红染色法、气相色谱法及热化学分析等方法探究热风干燥和真空冷冻干燥对3种不同来源恰玛古多糖结构、理化特性及体外抗氧化活性的影响。结果表明,热风干燥下多糖提取率(3.47±0.25)%和多糖含量(24.20±4.86)%普遍更好。恰玛古多糖主要由阿拉伯糖、葡萄糖及半乳糖组成,且不同干燥方式对恰玛古多糖的一级结构影响较大,对其高级结构影响较小。热化学分析结果显示热风干燥下热稳定性更强。真空冷冻干燥下恰玛古多糖的乳化性(72.76±0.30) m2/g和乳化稳定性(17.75±0.92) min更好且均高于市售蛋白(29.40 m2/g、14.90 min),热风干燥下的恰玛古多糖持水性(89.75±0.97) g/g和持油性(45.47±0.78) g/g普遍更好。热风干燥获得的恰玛古多糖对羟基自由基和ABTS+自由基清除率最高,半数抑制浓度 (half maximal inhibitory concentration ,IC??)为1.097 mg/mL和0.467 9 mg/mL,表明热风干燥是获得高品质恰玛古多糖的较好处理方式。
Abstract:To solve the problem of selecting the drying method of raw materials in the production process of Brassica rapa L. polysaccharide, polysaccharide was prepared by water extraction and alcohol precipitation with ′small ginseng′ Brassica rapa L. from different regions. The effects of hot air drying and vacuum freezedrying on the structure, physicochemical properties, and antioxidant activity of Brassica rapa L. polysaccharides from three different sources were investigated by ultraviolet absorption spectrometry, Fourier infrared spectroscopy, Congo red staining, gas chromatography,and thermochemical analysis. The results showed that the extraction yield (3.47±0.25)% and polysaccharide content (24.20±4.86)% were generally higher under hot air drying. Brassica rapa L. polysaccharide is mainly composed of arabinose, glucose, and galactose. The primary structure of Brassica rapa L. polysaccharide was significantly affected by different drying methods, but the higher structure was less affected. The thermochemical analysis results showed that the thermal stability was stronger under hot air drying. The emulsification property (72.76±0.30) m2/g and emulsion stability(17.72±0.92) min of Brassica rapa L. polysaccharide under vacuum freeze drying were better than those of commercial protein (29.40 m2/g and 14.90 min). The water vetention (89.75±0.97) g/g and oil retention(45.47±0.78) g/g of Brassica rapa L. polysaccharides under hot air drying was generally better. The scavenging rates of hydroxyl radical and ABTS+ radical of Brassica rapa L. polysaccharide obtained by hot air drying were the highest, with half maximal inhibitory concentration (IC??) of 1.097 mg/mL and 0.467 9 mg/mL. The results indicated that hot air drying was a better way to obtain high-quality Brassica rapa L. polysaccharides.
keywords: Brassica rapa L. polysaccharide structural characterization physicochemical property drying
文章编号:202523005 中图分类号: 文献标志码:
基金项目:新疆维吾尔自治区重点研发计划项目(2022B02037-2)
| Author Name | Affiliation |
| YUAN Miaolong | College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China |
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