Abstract:With the mycelia of?Agaricus?blazei?Murill as the material，the liquid fermentation medium formulation was optimized by single factor experiments and response surface design. The results showed that the most suitable carbon source，nitrogen source，and inoculum amount for the growth of?A.?blazei?mycelia were glucose，yeast extract powder，and 10%，respectively. Furthermore，with the mycelial biomass as the response value，the medium formulation was optimized as 32.894 g/L glucose，3.773 g/L yeast extract powder，and 10.434% inoculum amount according to the Box-Behnken design. The measured mycelial biomass in the optimal medium was 11.080 mg/mL，which was consistent with the theoretical value of 11.056 mg/mL under the same culture conditions.