Abstract:A simulated gastrointestinal digestion model was established for rye pollen peptides in vitro，and the relative molecular weight distribution and the antioxidant and blood glucose-lowering activities of rye pollen peptides were measured before and after simulated digestion. The results showed that the relative rye pollen peptides with the molecular weights lower than 1 kDa accounted for 86.09%. After simulated gastric and intestinal digestion，the content of peptides with molecular weights＜1 kDa increased by 8.72% and 12.19%，respectively. The simulated gastrointestinal digestion in vitro significantly enhanced the antioxidant activity of rye pollen peptides. Specifically，the simulated gastrointestinal digestion of rye pollen peptides at 6 mg/mL increased the DPPH and ABTS+ free radical scavenging activities by 14.13% and 10.02%，respectively. Rye pollen peptides and the gastric fluid and gastrointestinal fluid containing the digested peptides effectively inhibited the activities of α-amylase，α-glucosidase，and dipeptidyl peptidase IV （DPPIV） while significantly increasing the anti-advanced glycation end products （AGEs） activity.