Abstract:By measuring the content of indicators such as water， protein， polysaccharides， vitamin B1， vitamin B2， mineral elements， and amino acids of Morchella esculenta， this study explored the effects of three cultivation methods： greenhouse， cave， and under-forest， on the main nutritional components of Morchella esculenta. The results showed that Morchella esculenta contained rich nutrients under three cultivation conditions，but the content varied. The content of water and polysaccharides in Morchella esculenta was the highest under cave cultivation； the content of protein， vitamin B1， and vitamin B2 in Morchella esculenta was the highest under greenhouse cultivation； under all three cultivation conditions， Morchella esculenta was rich in mineral elements， among which phosphorus， iron， and copper had a high index of nutrition quality （INQ）， indicating that Morchella esculenta under three cultivation conditions could be used as preferred food for humans to supplement phosphorus， iron， and copper； Morchella esculenta grown under three cultivation conditions was rich in 18 amino acids， including eight essential amino acids for the human body. The evaluation of the amino acid pattern by Food and Agriculture Organization of the United Nations （FAO）/World Health Organization（WHO）， the ratio of amino acid （RAA）， the ratio coefficient of amino acid （RC）， and the score of RC（SRC） showed that Morchella esculenta grown under three cultivation conditions was a high-quality variety and contained high-quality proteins. The evaluation of the FAO/WHO pattern showed that Morchella esculenta grown in greenhouses was superior to that under other cultivation conditions， and the evaluation of RAA， RC，and SRC showed that Morchella esculenta grown in caves had the highest nutritional value among the three cultivation methods.