Abstract:A Shihu Gegen beverage with hepatoprotective effect was developed with Puerariae lobatae Radix（Gegen），Dendrobii officinalis Caulis （Shihu），Coicis Semen，Citri reticulatae Pericarpium，Hoveniae Semen，Chrysanthemi morifolit Flos，Glycyrrhizae Radix et Rhizoma，Menthae haplocalycis Herba，and Crataegi Fructus as the main raw materials. Its hepatoprotective effect was investigated via a mouse model of acute alcoholic liver injury. The results demonstrated that the Shihu Gegen beverage increased the body mass，reduced the liver index，and alleviated hepatocyte vacuolation and excessive nuclear division in the liver of the mouse model. Furthermore，the beverage decreased the activities of aspartate aminotransferase and alanine aminotransferase，lowered the levels of low-density lipoprotein，triglycerides，cholesterol，and malondialdehyde，and enhanced the levels of superoxide dismutase and glutathione peroxidase in the liver tissue. Analysis of the gut microbiota revealed that the beverage ameliorated the gut microbiota dysbiosis caused by 50% ethanol exposure，improving the abundance of Burkholderia，Spirillum，Verrucomicrobium，Lactobacillus，and Erysipelothrix.These findings suggested that the Shihu Gegen beverage had the potential to be developed into a functional health food product for alleviating 50% ethanol-induced liver injury.