Abstract:To establish a method for determining hexanoic acid content in honey by solid phase extraction-gas chromatography/mass spectrometry（SPE-GC/MS），honey samples were dissolved in deionized water，and the pH was adjusted to 8.0±0.5.The Strata-X-A solid-phase extraction column was used to enrich and purify hexanoic acid.The sample solution was eluted with a 2% formic acid-methanol solution，dried under a nitrogen stream，and redissolved with 2 mL of 50% methanol-water solution.Finally，hexanoic acid was determined by GC-MS.The results showed that hexanoic acid exhibited good linearity in the concentration range of 0.1-10 mg/L（R2=0.999 8）.The limit of detection（LOD）of the method was 0.06 mg/kg，and the limit of quantification（LOQ）was 0.20 mg/kg.Three spiking levels（0.2，0.4 mg/kg，and 2.0 mg/kg）were tested in blank samples，with average recoveries ranging from 96.8% to 107.6% and relative standard deviations ranging from 0.8% to 4.1%（n=6）.By using this method，16 samples of Leucosceptrum canum Smith honey and 61 samples of other honey varieties were analyzed.It was found that hexanoic acid was exclusively detected in all Leucosceptrum canum Smith honey samples，with concentrations ranging from 0.30 mg/kg to 4.14 mg/kg.Therefore，this method is easy to operate，highly sensitive，and accurate，making it suitable for determining hexanoic acid content in honey.Meanwhile，hexanoic acid can serve as a potential characteristic component of Leucosceptrum canum Smith honey for its authenticity identification and quality evaluation.