Abstract:With the increase in freshwater fish production and sales，the production of by-products such as fish bones，fins，skin，and viscera has also increased. These fish by-products contain abundant proteins and can be used to prepare active peptides with unique physicochemical properties and biological activities. The sources，preparation methods，and biological activities of bioactive peptides，as the by-products of freshwater fish processing，were reviewed. In addition，the problems and shortcomings encountered in the current research on bioactive peptides were summarized. The review was expected to provide ideas for further research and development of bioactive peptides in freshwater fish.