Abstract:To investigate the effects and mechanisms of dietary fiber of fermented black rice on hepatic lipid deposition in mice with non-alcoholic fatty liver disease（NAFLD）. An NAFLD mouse model was established using a methionine and choline deficiency （MCD） diet. The mice were treated with low-dose （2.5 g/kg bw）and high-dose （5.0 g/kg bw） dietary fiber of fermented black rice. After four weeks， liver histopathology was examined， and serological biomarkers， including alanine aminotransferase（ALT） and aspartate aminotransferase （AST） levels， were measured. Quantitative polymerase chain reaction （qPCR） was used to analyze the mRNA expression of genes related to lipid metabolism and transport， including ACACA， FASN， TGR5，CPT1α， FABP1， CD36， APOB， MTTP， and FXR. The results showed that in the low-dose group， there was a slight improvement in the pathological morphology of the mouse liver， while the changes in serological biomarkers and the mRNA expression levels of enzymes related to lipid metabolism and transport were not significant. In contrast， the high-dose group exhibited a marked improvement in the pathological morphology of the mouse liver， a significant reduction in serological biomarkers （p＜0.05）， and a significant upregulation in the mRNA expression of enzymes associated with lipid metabolism and transport（p＜0.05）. Hence， high-dose dietary fiber of fermented black rice could ameliorate NAFLD， which may be associated with its upregulation of signaling pathways related to lipid metabolism and transport genes.