Abstract:This study established an efficient and accurate ultra performance liquid chromatography （UPLC）method for the determination of biogenic amines in soy sauce. The composition of soy sauce is complex，and there are many factors affecting the detection of biogenic amine content. In order to obtain a more accurate biogenic amine content，different acidic extractants，extraction methods and time，as well as derivative temperature，derivative time and derivative agent volume were selected as the conditions for the optimization test of soy sauce pretreatment. A UPLC method for the determination of tryptamine，phenylethylamine，putrescine，cadaverine，histamine，tyramine，spermidine and spermine in soy sauce was established by multiple experiments.The results showed that the optimum conditions were as follows 5% trichloroacetic acid as extractant，ultrasonicassisted extraction for 30 min，and derivatization at 42 ℃ for 45 min with 1 mL derivatization agent. The eight biogenic amines could be separated within 26 min，and the linearity was good in the range of 2.5-50.0 mg/L.The correlation coefficient R2 was 0.996 4-0.999 5，the detection limit was 0.02-0.15 mg/L，and the limit of quantitation was 0.10-0.75 mg/L. The average spiked recovery of the samples was 72.00%-122.53%，and the relative standard deviation （RSD） was 0.78%-12.23%. The methodological test showed that the method established in this study had high sensitivity and accurate results，which could meet the requirements of qualitative and quantitative analysis of biogenic amines in soy sauce.