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投稿时间：2024-03-21
投稿时间：2024-03-21
中文摘要: 采用绿色低共熔溶剂提取金顶侧耳中天然麦角硫因，筛选低共熔溶剂氢键受体和供体的配方，借助响应面优化最佳提取工艺，并考察该工艺下麦角硫因的抗氧化活性。结果表明，金顶侧耳中麦角硫因的最适低共熔体系由氯化胆碱和尿素组成，物质的量比为1∶2，含水率为30%。最佳提取工艺条件为料液比1∶35（g/mL）、提取温度71 ℃、提取时间90 min，此条件下麦角硫因提取量为2.70 mg/g，经验证与预测值相对标准偏差为1.48%；该工艺下的麦角硫因具有较高的清除羟自由基、DPPH 自由基、ABTS＋自由基能力及氧化自由基吸收能力。
Abstract:The natural ergothioneine was extracted from the Pleurotus citrinopileatus Sing by green deep eutectic solvents. The formula of eutectic solvents′ hydrogen bonding acceptors and donors were screened. Then，the extraction process was optimized by the response surface method，followed by an investigation of the ergothioneine′s antioxidant properties under such conditions.The results showed that the most suitable deep eutectic system for ergothioneine extraction from Pleurotus citrinopileatus Sing was composed of choline chloride and urea with a molar ratio of 1∶2 and a moisture content of 30%.The ideal extraction process was as follows：a material-liquid ratio of 1∶35（g/mL），an extraction temperature of 71 ℃，and an extraction time of 90 min.Under these conditions，the extraction amount of ergothioneine could reach 2.70 mg/g，with a relative error of only 1.48% compared to the predicted value. Additionally，the extracted ergothioneine presented satisfactory scavenging capacities against hydroxyl，DPPH，and ABTS+radicals.
keywords: Pleurotus citrinopileatus Sing ergothioneine deep eutectic solvents response surface method antioxidant activity
文章编号：202513011 中图分类号： 文献标志码：
基金项目:浙江省“三农九方”科技协作计划项目（2025SNJF037）；国家自然科学基金项目（31571795）；浙江省省院合作重大项目（2024SY08）
| 作者 | 单位 |
| 夏张晨1，孟晓慧1，程俊文1，王衍彬1，王丽玲1，杨开2，毛延卿3，贺亮1* | 1.浙江省林业科学研究院森林食品资源利用与质量控制国家林业局重点实验室,浙江杭州310023；2.浙江工业大学食品科学与工程学院,浙江杭州310014；3.杭州众芝康菇生物技术有限公司,浙江杭州310000 |
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