Abstract:The synergistic effects of ethanol extracted from Stigma maydis and Lentinus edodes on scavenging hydroxyl free radicals，DPPH free radicals，and ABTS+free radicals were analyzed by using the Chou‐Talalay method and the isoradiation method. The results showed that when the free radical scavenging rate was 50%，ethanol extracted from Stigma maydis and Lentinus edodes showed a synergistic effect on the scavenging of three free radicals，and different compounding ratios had an impact on the synergistic effect. Overall，the 1∶1 compounding ratio had the best synergistic effect.The main contributor to the synergistic effect was the alcohol extract from Stigma maydis.When alcohol extracts from Stigma maydis and Lentinus edodes were used in com‐bination，they all had the effect of reducing their respective doses，among which the dose reduction effect of the alcohol extract from Lentinus edodes was more significant than that from Stigma maydis. Their synergistic per‐formance in scavenging three types of free radicals is slightly different.The best synergistic effect was observed in scavenging DPPH free radicals，followed by ABTS+free radicals and hydroxyl free radicals.