Abstract:This study investigated the effect of an aqueous extract of Poria cocos and Coix seed on obese mice induced by a high-fat diet （HFD）. Mice were fed an HFD to establish an obesity model. They were administered drugs for 12 weeks，and their body mass，diet，and drinking water were monitored every week. After the experiment，the physiological and biochemical indexes of mice were detected. Western blotting assay was used to detect the expression of proteins related to lipolysis and browning in adipose tissue. The results showed that compared with the HFD group，the aqueous extract of Poria cocos and Coix seed significantly inhibited the increase in the body weight and fat mass in mice，reduced blood lipid levels，improved the disorders of glucose and lipid metabolism，and increased the relative expression levels of AMP-activated protein kinase（AMPK），uncoupling protein 1 （UCP-1），peroxisome proliferator-activated receptor γ coactivator-1α （PGC-1α），adipose triglyceride lipase （ATGL），and hormone-sensitive lipase （HSL）. In conclusion，the aqueous extract of Poria cocos and Coix seed could reduce body weight，reduce fat accumulation，and improve glucose and lipid metabolism disorders in mice，and its mechanism of action was related to promoting lipolysis and white fat browning.