Abstract:To investigate the antioxidant active components and mechanisms of Astragalus root extract，effective components were extracted using four organic solvents：petroleum ether，ethyl acetate，chloroform，and nbutanol and detected using high performance liquid chromatography（HPLC）. The antioxidant effects were studied based on network pharmacology. Multiple online databases were employed to identify common targets between Astragalus and antioxidants. Protein-protein interaction networks（PPI）were constructed，and gene ontology（GO）enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG）pathway enrichment analysis were performed.The results showed that all Astragalus root extracts had certain scavenging abilities against ·OH，O2-·，and DPPH· radicals. The ethyl acetate and chloroform extracts of Astragalus exhibited better scavenging effects，with kaempferol，oleanolic acid，and gallic acid identified as the main antioxidant active components. A total of 136 antioxidant targets for Astragalus were screened through online databases. GO enrichment indicated that these targets were mostly related to responses to inorganic substances，hormones，and external stimuli. KEGG enrichment analysis revealed that these targets were associated with cancer pathways，lipid and atherosclerosis，and the hypoxia-inducible factor signaling pathway. The molecular docking results showed better antioxidant effect of the extracted active ingredients.