Abstract:The adhesion and colonization of probiotics on human intestinal cells were an important prerequisite for giving full play to the probiotic effects. The interaction between the surface components as adhesion factors and sites of probiotics was the key link affecting the adhesion and colonization of probiotics. The receptors interacting with adhesion factors at the adhesion sites included not only some substances in the mucous layer but also extracellular substances secreted by intestinal cells. The protein and non-protein adhesion factors on probiotic surface have adhesion specificity，and they can promote the adhesion and colonization of probiotics by regulating the expression of adhesion factors. The recent studies about intestinal adhesion sites were reviewed，and the relationship between the expression of adhesion factors and adhesion during biofilm formation was analyzed，which would provide a reference for giving full play to the role of adhesion factors in the human body and lay a theoretical foundation for exploring the influence of biofilm formation on adhesion.