Abstract:The lack of a theoretical model and quantitative basis for spontaneous modified atmosphere packaging（MAP）of broccoli leads to errors or failures in packaging system design.The 'excellent cold-resistant'broccoli，a specialty plateau summer vegetable of Gansu Province，was taken as the research object，and a closed system method was used to calculate the respiration rate of broccoli at different temperatures（0，5，10，15 ℃，and 20 ℃）.By considering the ease of use，consistency with existing respiration data，and comprehensiveness of factors covered，the respiration rate model of broccoli was built based on the non-competitive and chemical kinetics theories of enzyme kinetics.The coefficients of determination for both models at all temperatures were greater than 0.90.Through the comparison of model-related statistical indexes and predicted and experimental values，the model prediction accuracy and generalization capability were tested.The results showed that the non-competitive enzyme kinetics model was selected to characterize the actual respiration rate characteristics of broccoli.With the storage temperature at 12 ℃as an example，the established non-competitive enzyme kinetics respiration rate model was combined with the gas exchange model of MAP to develop a prediction model for the spontaneous MAP storage of broccoli.The prediction model was used to design the parameters of a spontaneous MAP system for broccoli at a storage temperature of 12 ℃，illustrating the application of the model.