Abstract:The basic principles，process characteristics，and the application of enzyme-assisted extraction techniques in improving the extraction yields of various types of natural active substances were summarized in this article，such as polysaccharides，flavonoids，lipids，alkaloids，saponins，and organic acids. Additionally，the differences in quality and biological activity of natural active substances between enzyme-assisted extraction and traditional methods were compared.Enzyme-assisted extraction has evolved into a method that employs a combination of multiple techniques，and is a promising method for extracting active substances from plants. In light of the technical challenges and high production costs associated with enzyme-assisted extraction，feasible solutions were proposed.