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食品研究与开发:2024,45(9):104-110
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铜藻多酚的提取工艺及其稳定性和抑制酪氨酸酶活性
(1.自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门 361005;2.福建农林大学食品科学学院,福建福州 350001;3.厦门海洋职业技术学院海洋生物学院,福建厦门 361100)
Extraction Process,Stability,and Inhibitory Effect on Tyrosinase of Polyphenols from Sargassum horneri
(1.Technical Innovation Center for Utilization of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China;2.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350001,Fujian,China;3.College of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,Fujian,China)
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投稿时间:2022-12-20    
中文摘要: 对铜藻多酚提取工艺、稳定性及其对酪氨酸酶活性的抑制作用进行研究。以新鲜铜藻为原料提取铜藻多酚,以铜藻多酚提取率为指标,考察乙醇体积分数、提取时间、液料比对铜藻多酚提取率的影响,并根据Box-Behnken试验设计对铜藻多酚的提取条件进行优化,得到铜藻多酚的最佳提取条件:乙醇体积分数40%、超声时间53 min、液料比为60∶1(mL/g),在该条件下所制备的铜藻多酚具有较高的热和光照稳定性。以左旋酪氨酸和左旋多巴为底物,铜藻多酚浓度为700 μg/mL 时,对酪氨酸酶活性的抑制率分别为92.68%和39.18%。
中文关键词: 铜藻  多酚  提取工艺  稳定性  酪氨酸酶
Abstract:The extraction process,stability,and inhibitory effect on tyrosinase activity of polyphenols from Sargassum horneri were studied. Polyphenols were extracted from fresh S. horneri. The effects of ethanol volume fraction,extraction time and liquid-to-solid ratio on the yield of polyphenols from S. horneri were investigated. Box-Behnken design was then employed to optimize the extraction conditions. The optimum extraction conditions of S.horneri polyphenols were as follows:ethanol volume fraction of 40%,ultrasonic time of 53 min,and liquid-to-solid ratio of 60∶1(mL/g). The S. horneri polyphenols prepared under the optimum conditions had high thermal and light stability.With L-tyrosine and L-dopa as substrates,the inhibition rates of 700 μg/mL S.horneri polyphenols on tyrosinase activity were 92.68% and 39.18%,respectively.
文章编号:202409015     中图分类号:    文献标志码:
基金项目:福建省科技计划项目(2021N0029);自然资源部第三海洋研究所基本科研业务费专项资金资助项目(2022006);福建省海洋经济发展资金项目(FJHJF-L-2021-4);厦门海洋研究开发院共建项目(K210103)
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