Abstract:The extraction and enrichment process of phospholipids from Antarctic krill（Euphausia superba）using two-liquid-phase system of hexane-ethanol-water was investigated in this study. Ethanol was used to extract total lipids from Antarctic krill，and the yield of total lipids was taken as the evaluation index.The effects of material-liquid ratio，extraction time，extraction temperature and extraction times on the extraction efficiency of total lipids from Antarctic krill were firstly evaluated.Subsequently，the optimal condition for enriching phospholipids from the total lipids of Antarctic krill using the two-liquid-phase system of hexane-ethanolwater was optimized with lipid yield，phospholipid content and phospholipid yield as the evaluation indexes.The results showed that the optimal conditions for extracting total lipids from Antarctic krill using ethanol were a material-liquid ratio of 1∶3（g/mL），extraction time of 3 h，extraction temperature of 40 ℃，and extraction 3 times. Under these conditions，the yield of total lipids from Antarctic krill was（4.69 ± 0.13）%. The optimal volume ratio of hexane-ethanol-water for enriching phospholipids from the total lipids of Antarctic krill was 0.275∶0.600∶0.125. The phospholipid content in the obtained Antarctic krill phospholipids reached up to（85.46±0.31）%.The main component of Antarctic krill phospholipids was phosphatidylcholine，and the total amount of eicosapentaenoic acid（EPA）and docosahexaenoic acid（DHA）in phospholipids accounted for（53.39±0.76）% of total fatty acid content.