Abstract:It was extracted from pomegranate seeds by supercritical CO2,and the composition and content of fatty acids and triglycerides were analyzed. The enrichment of punicic acid methyl ester by urea inclusion was studied by single factor and orthogonal optimization experiments.Compared with organic solvent extraction,supercritical CO2 was more favorable to obtain pomegranate seed oil containing high punicic acid,where 14 triglycerides were detected.The optimal conditions for the enrichment of punicic acid methyl ester by urea inclusion were as follows:the inclusion temperature 5 ℃,inclusion time 20 h,mass ratio of urea to methyl ester 1∶1,and mass ratio of urea to 95% ethanol 1∶5.Under these conditions,the content of punicic acid methyl ester was 78.82%.