Abstract:With the development of high-throughput genomics technology，metabolomics has become an important means of exploring the biosynthesis pathways and molecular regulatory mechanisms of flavor compounds in fruits and vegetables. In this paper，from the perspectives of variety origin，developmental stage，processing mode and cultivation mode，the latest research on the use of metabolomics in the analysis of flavor compounds in fruits and vegetables was sorted out and summarized，and it was concluded that high-throughput metabolomics could be used to assess the changes of flavor-related metabolites in fruits and vegetables，and be widely used in the cultivation of fruits and vegetables，the quality assessment in processing and storage，the development of new products，and the optimization of the breeding and so on. At the same time，using metabolomics technology can reveal the important chemical components and influencing factors of volatile and nonvolatile flavor presenting substances in fruits and vegetables，so as to provide a theoretical basis for the study of gene function in the formation of flavor compounds in fruits and vegetables，and to provide new ideas for the development of flavor and quality control of fruits and vegetables，genetic breeding，and food processing.