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投稿时间：2023-04-03
投稿时间：2023-04-03
中文摘要: 为建立试验鸡肉中水飞蓟宾含量的高效液相色谱（high performance liquid chromatography，HPLC）检测方法，以乙酸乙酯为提取剂从鸡肉匀浆中提取水飞蓟宾后，采用C18 色谱柱，在流动相为水∶甲醇=47∶53（体积比）、柱温30 ℃、流速0.65 mL/min、检测波长288 nm、进样量20 μL 的条件下进行分离检测。结果显示，水飞蓟宾标准品浓度在0.1~5.0 μg/mL 时线性关系良好，标准曲线方程为y=95.16x-0.875 3，相关系数R2=0.999 6，检测试验鸡肉中水飞蓟宾的平均回收率为88.45%~104.34%，相对标准偏差（relative standard deviation，RSD）为1.85%~2.94%。结果表明，该方法简单易行，精密度较高，回收率良好，可用于试验鸡肉中水飞蓟宾含量的测定。
Abstract:In order to establish a high performance liquid chromatography（HPLC）method for the determination of silybin in chicken used in the experiment，ethyl acetate was used as the extraction agent to extract silybin from chicken homogenate，and C18 chromatographic column was used. The separation and detection were performed under optimized conditions such as mobile phase of water∶methanol = 47∶53（volume ratio），column temperature of 30 ℃，flow rate of 0.65 mL/min，detection wavelength of 288 nm，and sample size of 20 μL. The results showed that the linear relationship was good when the concentration of silybin standard product was 0.1-5.0 μg/mL. The standard curve equation was y = 95.16x-0.875 3，and the correlation coefficient R2 = 0.999 6. The average recovery of silybin in the chicken was 88.45%-104.34%，and the relative standard deviation（RSD）ranged from 1.85% to 2.94%. The results showed that the method was simple and feasible. It had high precision and good recovery and could be used for the determination of silybin in chicken.
keywords: high performance liquid chromatography experimental chicken silybin content determination functional food
文章编号：202405025 中图分类号： 文献标志码：
基金项目:天津市科技计划项目（22YDTPJC00990、21YDTPJC00580、18ZXBFNC00310）；天津市教委科研计划项目（2021KJ108）；天津市“131”创新型人才团队项目（20180318）
| 作者 | 单位 |
| 侯泽桦1，李羡钰1，孙雯可1，谢艾伶1，刘全瑞1，刘鼎阔2，于晓雪1 *，李留安1 * | 1.天津农学院 动物科学与动物医学学院，天津市农业动物繁育与健康养殖重点实验室，天津 300392；2.鼎正新兴生物技术（天津）有限公司 天津市生物饲料添加剂企业重点实验室，天津 300383 |
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