Abstract:In order to investigate the effect of Lactobacillus plantarum CCFM8661 on the intestinal flora of mice，mice were acclimatized for one week，gavaged with low（1.6 × 106 CFU/mouse），medium（1.6 ×107 CFU/mouse），and high（1.6 × 108 CFU/mouse）doses of Lactobacillus plantarum CCFM8661，and then intervened for 14 d. The changes of bifidobacteria，lactobacillus，enterococcus，Enterobacteriaceae，and Clostridium perfringens in the mouse stool were detected before and after the intervention. Histopathology，intestinal barriers，intestinal permeability，and short-chain fatty acids were measured to evaluate the regulatory effects of Lactobacillus plantarum CCFM8661 on intestinal microorganisms and intestinal barriers. The results showed that Lactobacillus plantarum CCFM8661 could increase the content of bifidobacterium and Lactobacillus and inhibit the proliferation of Clostridium perfringens. Meanwhile，feeding Lactobacillus plantarum CCFM8661 could significantly change the intestinal permeability of mice，reduce the content of lipopolysaccharide（LPS）and D-lactic acid in the intestinal tract，enhance the intestinal barrier，and raise the content of short-chain fatty acids（acetic，propionic，and butyric acids）in the intestinal tract. In conclusion，Lactobacillus plantarum CCFM8661 was able to regulate the intestinal flora and had a protective effect on the intestinal barrier.