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投稿时间:2023-08-21
投稿时间:2023-08-21
中文摘要: 为开发一种感官品质优良且富含植物乳杆菌的普洱熟茶,将植物乳杆菌(Lactobacillus plantarum)PET003 在自然发酵第20 天时接种于茶样中,进行接菌强化发酵普洱熟茶。细菌16S rRNA 扩增子测序发现,接菌强化发酵茶样中乳杆菌目(Lactobacillales)、微球菌目(Micrococcales)的相对丰度和植物乳杆菌属的DNA 序列片段数较对照样品(未接种植物乳杆菌)增加。与对照样品比较,接菌发酵茶样中可溶性糖、茶褐素、茶多酚、咖啡碱、儿茶素、表儿茶素、没食子儿茶素、儿茶素没食子酸酯含量显著升高(P<0.05);感官审评发现接菌发酵茶样汤色红褐明亮,茶汤甜度和醇厚度增加。
Abstract:To develop a kind of ripe Pu-erh tea with excellent sensory quality and rich in Lactobacillus plantarum(L. plantarum),L. plantarum PET003 was inoculated into the tea leaves under natural fermentation at 20 d for enhanced fermentation of ripe Pu-erh tea. Bacterial 16S rDNA amplicon sequencing showed that the relative abundance of Lactobacillales and Micrococcales,as well as the number of L. plantarum reads in fermented tea samples were increased compared with the control samples(without inoculation with L. plantarum). The content of soluble sugar,theabrownin,tea polyphenols,caffeine,catechin,epicatechin,gallocatechin,and catechin gallate in enhanced fermented tea samples was significantly higher than that in the control samples(P<0.05). Sensory evaluation showed that the color of fermented tea samples was reddish brown,and the sweetness and mellowness of the tea soup were increased.
keywords: ripe Pu-erh tea Lactobacillus plantarum enhanced fermentation chemical composition microbial community
文章编号:202405004 中图分类号: 文献标志码:
基金项目:国家自然科学基金资助项目(32160728);云南省科技厅科技计划项目(202104BI090008);云南农业大学第十六届学生科技创新创业行动基金项目(2023N099);中央引导地方科技发展资金项目(202207AD110008)
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