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投稿时间：2023-08-10
投稿时间：2023-08-10
中文摘要: 利用超声波、吐温-80、丙酮和甲苯等改变大黄欧文氏菌DXW-62 的细胞膜通透性，研究其对异麦芽酮糖产率的影响。结果显示：90 W 超声波处理1 min 后，与空白对照组相比，异麦芽酮糖产率达到80.0% 以上的时间由4.0 h 缩短为3.0 h；添加浓度为5.0%的吐温-80 处理后，异麦芽酮糖产率在2.0 h 时已达到了75.40%，与空白对照组相比，异麦芽酮糖产率达到最高值的时间由4.0 h 缩短为2.5 h；2.0%的丙酮处理后，在3.5 h 时异麦芽酮糖产率达到最大，为83.04%。低浓度的甲苯处理DXW-62 时的异麦芽酮糖产率无明显提高，高浓度的甲苯处理时异麦芽酮糖产率有明显下降。超声波和吐温-80 共同处理后异麦芽酮糖产率远低于对照组，经碘化丙啶染色后显红色荧光细胞数量最多。
Abstract:Ultrasonic wave，tween-80，acetone，toluene，and other methods were used to change the plasma membrane permeability of Erwinia rhubarb DXW-62，so as to understand its effect on the yield of isomaltulose.The results showed that after 90 W ultrasonic treatment for 1 min，the time for isomaltolose yield to reach more than 80.0% was shortened from 4.0 h to 3.0 h compared with the control group. After the addition of tween-80 with a concentration of 5.0%，the yield of isomaltulose reached 75.40% at 2.0 h. Compared with the control group，the time to reach the maximum isomaltulose yield was shortened from 4.0 h to 2.5 h.After 2.0% acetone treatment，the yield of isomaltulose reached a maximum of 83.04% at 3.5 h.The yield of isomaltulose of DXW-62 was not significantly increased under toluene treatment under a low concentration but significantly decreased under toluene treatment with a high concentration. The yield of isomaltulose after ultrasonic wave and tween-80 treatment was much lower than that of the control group，and the amount of red fluorescence cell was the highest after propyl iodide staining.
文章编号：202404003 中图分类号： 文献标志码：
基金项目:2022年度许昌学院“揭榜挂帅”项目（2022JBGS10）；2022年河南工业大学研究生教育改革与质量提升工程项目（HAUTYJS2022JD02）
| Author Name | Affiliation |
| LI Xiaoya，WU Tiantian，WANG Hongling，GE Wenpei，LIU Na* | College of Bioengineering，Henan University of Technology，Zhengzhou 450001，Henan，China |
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