Abstract:The synergistic regulating effects and mechanism of the compound extracts of lotus leaf，hawthorn，and Eucommia ulmoides leaf on lipid metabolism were compared with those of single plant extracts.The supercritical extraction technique was employed to obtain the extracts of lotus leaf，hawthorn，and E. ulmoides leaf and the compound extracts.The Wistar rats fed with a high-fat diet were administrated with the extracts respectively. The liver morphology was observed，and the expression levels of genes in lipid metabolism were determined. The results showed that the compounded extracts reduced the body mass and improved the lipid metabolism of rats（P＜0.05）compared with the single extract groups and the high fat model group. Hematoxylineosin（HE）staining results showed that the compound extracts significantly reduced the lipid droplet vacuoles and improved the cellular morphology of the liver tissue compared with the high fat model group and single extract groups.The results of molecular tests showed that compared with single extracts，the compound extracts upregulated the expression levels of peroxisome proliferator-activated receptor-alpha（PPARα）and cytochrome P450 family 7 subfamily A member 1（CYP7A1）in the liver（P＜0.05）. In addition，the compound extracts down-regulated the expression level of niemann-pick C1-like 1（NPC1L1）and up-regulated the expression level of ATP-binding cassette subfamily G member 5（ABCG5）in the small intestine（P＜0.05）.In conclusion，the compound extracts of lotus leaf，hawthorn，and E. ulmoides leaf could lower the blood lipid level in rats by up-regulating the expression of PPARα/CYP7A1 in the liver and down-regulating the expression level of NPC1L1 and elevating the expression level of ABCG5 in the small intestine. Moreover，the compound extracts with synergy effects outperformed single extracts.