Abstract:Insoluble dietary fiber（IDF）was extracted from bamboo shoot shells by chemical method.High performance liquid gel permeation，ion chromatography，scanning electron microscopy and Fourier infrared spectroscopy were employed to study the structural characteristics，and its water-holding，swelling and oil-holding capacity，and adsorption of cholesterol and bile salts were determined.Its impacts on intestinal microorganism were evaluated by in vitro digestion model.The results showed that IDF from bamboo shoot shells had good hydration properties with water-holding power and swelling power of 11.26 g/g and 8.20 mL/g as well as oil-holding power of 7.56 g/g.The extracted IDF was mainly composed of xylose，accounting for 62.70%.It had an irregular lamellar structure with many wrinkled surfaces，which made it possess good adsorption of water and glucose molecules.The IDF had a better ability to bind cholesterol in the small intestine than in the gastric juice，and had an excellent glucose adsorption capacity.In vitro fermentation revealed that IDF from bamboo shoot shells increased the abundance of Bacteroides and Caulobacteria，indicating a potential of regulating the intestinal flora.