Abstract:As an important natural product，polyphenols have a variety of potential beneficial effects.The structure-activity relationship between polyphenols and proteins has been the focus of research in the field of natural product chemistry.Polyphenols and proteins can generate a series of covalent and non-covalent interactions，which are widely used in food，medicine，and biology.Meanwhile，the structure of polyphenols largely influences their interaction with proteins.This paper briefly reviewed the mechanisms of polyphenolic-protein interactions and common research methods，reviewed the structure-protein interactions of common and important polyphenolic compounds，i.e.，phenolic acids，flavonoids，catechins，astragalosides，and finally provided an outlook on the future direction of conformational studies of polyphenolic-protein interactions.