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投稿时间：2022-12-07
投稿时间：2022-12-07
中文摘要: 采用沉淀聚合法，以鹰嘴豆芽素A（biochanin A，BCA）为模板分子，1-乙烯基吡啶（1-vinylimidazole，1-viny）为功能单体，二甲基丙烯酸乙二醇酯（ethylene glycol dimethacrylate，EDMA）为交联剂，制备鹰嘴豆芽素A分子印迹聚合物（biochanin A molecularly imprinted polymers，BCA-MIPs），将其用于分离降香叶中BCA。结果表明：BCA 与1-viny 之间通过氢键相互作用；当BCA、1-viny 及EDMA 摩尔比为1∶2∶10 时，BCA-MIPs 最大吸附量为6.52 mg/g，且在340 ℃以内较稳定；斯卡查德分析显示BCA-MIPs 形成一种结合位点，吸附性能符合准二级动力学方程，且对BCA 选择性强；将BCA-MIPs 用于固相萃取的填料，上样后，经4.19 mL 甲醇洗涤，34.90 mL 甲醇与乙酸（体积比9∶1）洗脱，BCAMIPs 对降香叶中BCA 分离效果最佳，可重复利用9 次。
Abstract:The biochanin A molecularly imprinted polymers（BCA-MIPs）were prepared via the precipitation polymerization with biochanin A（BCA）as the template molecule，1-vinylimidazole（1-viny）as the functional monomer，and ethylene glycol dimethacrylate（EDMA）as the cross-linking agent.The prepared BCA-MIPs were then used as the solid phase extraction filler for the separation of BCA from Dalbergia odorifera leaves.The results showed that BCA interacted with 1-viny by hydrogen bonds.When the molar ratio of BCA，1-viny，and EDMA was 1∶2∶10，the obtained BCA-MIPs showed the maximum adsorption capacity of 6.52 mg/g and the thermal stability within 340 ℃.The Scatchard analysis showed that the BCA-MIPs formed a binding site and possessed a strong recognition ability for BCA，with the adsorption performance conforming to a pseudo-second-order kinetic equation.After the loading of BCA-MIPs，the sample was washed with 4.19 mL methanol and eluted with 34.90 mL methanol and acetic acid（volume ratio：9∶1）.The BCA-MIPs achieved the expected effect on the separation of BCA from D.odorifera leaves and could be reused for 9 times.
keywords: biochanin A molecularly imprinted polymer solid-phase extraction Dalbergia odorifera leaves molecular recognition
文章编号：202323011 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（82160733）；江西省教育厅科技项目（GJJ201203）；江西省卫生与健康委员会科技项目（202131043）
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