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投稿时间：2023-04-28
投稿时间：2023-04-28
中文摘要: 该文探究蹄叶橐吾黄酮（flavonoids from Ligularia fischeri，FLF）的最佳提取工艺并分析其抗氧化能力。以超声波辅助纤维素酶酶解法提取FLF，通过单因素试验、Plackett-Burman 和响应面优化试验得出FLF 最佳提取工艺，并对还原力、DPPH·清除率和ABTS+·清除率进行测定，探究FLF 体外抗氧化活性。结果表明，最佳提取工艺为纤维素酶添加量168 mg、酶解温度50 ℃、酶解时间51 min、料液比1∶25（g/mL）、超声功率190 W、提取时间74 min，此工艺下FLF得率为2.05%。FLF 具有一定的抗氧化能力，但弱于维生素C。超声波辅助纤维素酶酶解法可以有效提高FLF 的得率，得到的FLF 具有较优的抗氧化活性。
Abstract:To explore the optimal extraction process of flavonoids from Ligularia fischeri （FLF）and analyze its antioxidant capacity，FLF was extracted by ultrasound-assisted cellulase enzymatic hydrolysis.The optimal extraction process was obtained by single factor test，Plackett-Burman test and response surface optimization test.The antioxidant activity of FLF in vitro was investigated by the determination of reducing power，DPPH·and ABTS+·scavenging ability.The optimum extraction conditions were cellulase dosage of 168 mg，hydrolysis temperature of 50 ℃，hydrolysis time of 51 min，solid-liquid ratio of 1∶25 （g/mL），ultrasonic power of 190 W，and extraction time of 74 min to yield 2.05% FLF.FLF had antioxidant capacity which was worse than VC.Ultrasound-assisted cellulase enzymatic hydrolysis can effectively improve the yield of FLF and the extracted FLF has better antioxidant activity.
文章编号：202320020 中图分类号： 文献标志码：
基金项目:吉林省科技发展计划项目（20200708054YY）
| Author Name | Affiliation |
| WANG Mengmeng，CUI Jing'ai* | College of Food Science and Engineering，Jilin Agricultural University，Changchun 130118，Jilin，China |
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