Abstract:A short-term alcoholic steatohepatitis model（Gao-Binge model）was established to explore the effects of hyperin with low，moderate，or high dosages on alcoholic liver diseases induced by the alcoholic diet of mice.Levels of serum transaminase，triglyceride（TG），superoxide dismutase（SOD），and malondialdehyde（MDA）in liver tissue were measured.Liver histopathological changes were observed by hematoxylin and eosin staining（H&E staining）and oil red staining.Liver neutrophil infiltration was detected by immune-histochemical staining.Finally，the expression of inflammatory cytokines and chemokines in liver was analyzed by real-time quantitative polymerase chain reaction（RT-PCR）.The results showed that after feeding with an alcohol liquid diet，the mice in the model group developed steatohepatitis，while hyperin could significantly reduce the levels of serum transaminase and TG and the area of oil red staining，decrease the level of MDA in liver，and increase the activity of SOD enzyme.In addition，hyperin inhibited the expression of interleukin 6（IL-6）and tumor necrosis factor（TNF-α）in liver induced by an alcoholic diet and reduced neutrophil infiltration and chemokine expression in liver tissue.This suggested that hyperin could improve alcoholic steatohepatitis in mice by reducing the level of lipid peroxidation in the liver and inhibiting inflammation.