Abstract:Using Salmonella enteritidis as the study object，a visual detection method was developed based on nano-gold and a nucleic acid aptamer.By optimizing the aptamer concentration in the system，the detection limit，specificity，and optimal temperature of the nano-gold aptamer system were determined.Meanwhile，the rate of reclamation of the nano-gold aptamer was evaluated using artificially polluted samples.The results showed that this method specifically detected S.enteritidis and had no specific reaction to other foodborne pathogens.After optimizing the conditions，the minimum detection limit of S.enteritidis was 9.3×101 colony-forming units（CFU）/mL and it exhibited a good linear relationship in the range of 103-107 CFU/mL，with a linear formula of y=0.187 8x-0.146（R2=0.991 3）.The rate of reclamation of artificially contaminated samples was 93.68%-117.89%.The results showed that the detection of S.enteritidis by nucleic acid aptamer nano-gold colorimetry was simple and the results were visual.Other pathogenic bacteria may also by detected by adjusting the nucleic acid aptamer，which has significance for the widespread adoption of this method.