Abstract:The aim of this study was to establish a method for the determination of L-carnitine in chicken plasma by high-performance liquid chromatography（HPLC）.The samples were extracted with a mixture of acetonitrile and methanol（9∶1，volume ratio），vortexed，centrifuged，and diluted to a constant volume.After filtration with a 0.22 μm filter membrane，the samples were separated using an ACE Excel 5 Super C18 chromatographic column.The detection wavelength was 210 nm.The mobile phase was acetonitrile-5 mmol/L sodium heptanesulfonate（adding 0.1%phosphoric acid）aqueous solution（15∶85，volume ratio），the flow rate was 0.6 mL/min，the column temperature was 30 ℃，the detection wavelength was 210 nm，and the injection volume was 20 μL.The results showed that under this condition，the mass concentration of L-carnitine and its peak area had a good linear relationship in the range of 0.01-0.60 mg/mL，the correlation coefficient was 0.999 8，the detection limit was 0.005 mg/mL，and the limit of quantitation was 0.017 mg/mL.The average recovery rate was 97.393%-98.703%，and the relative standard deviation（RSD）was 0.8%（n=6）.Using this method，the L-carnitine peak in plasma was good and there was no impurity peak interference.The results showed that the method had low cost，simple operation and high recovery rate，which was suitable for the determination of L-carnitine in chicken plasma.