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投稿时间:2023-03-07
投稿时间:2023-03-07
中文摘要: 采用超声辅助提取法提取香菜中多酚,结合单因素试验和正交试验,确定香菜多酚最佳提取工艺。利用铁还原/抗氧化能力分析法测定香菜多酚提取液总抗氧化能力,用邻二氮菲法测定羟基自由基清除能力,通过滤纸片法测定香菜多酚的抑菌活性。研究结果表明,香菜多酚超声辅助提取的最佳条件为料液比1∶16(g/mL)、乙醇体积分数75%、提取时间60 min,最优条件下提取率为(13.11±0.19)mg/g。抗氧化试验结果表明,香菜多酚总抗氧化能力和羟基自由基清除率随香菜多酚浓度增大而增强,0.6 mg/mL 香菜多酚提取液的总抗氧化能力为(102±2)μmol/L,羟基自由基清除率为(60±1)%。抑菌试验结果表明,香菜多酚对大肠杆菌和金黄色葡萄球菌均有一定的抑制作用,且随着多酚浓度升高,抑菌作用增强。0.6 mg/mL 香菜多酚提取液对大肠杆菌进行活性测试的抑菌圈直径为(6.76±0.02)mm,对金黄色葡萄球菌活性测试的抑菌圈直径为(7.04±0.02)mm。
Abstract:The polyphenols in coriander were extracted using ultrasound-assisted extraction method,and the optimal extraction process for coriander polyphenols was determined through single-factor and orthogonal experiments.The total antioxidant capacity of coriander polyphenol extract was measured using the iron reduction/antioxidant capacity analysis method,and the hydroxyl radical scavenging capacity was determined using the o-phenanthroline method.The antibacterial activity of coriander polyphenols was determined using the filter paper sheet method.The results showed that the optimal conditions for ultrasound-assisted extraction of coriander polyphenols were as follows:the solid-to-liquid ratio of 1∶16(g/mL),ethanol volume fraction of 75%,and extraction time of 60 min.Under these conditions,the extraction yield was(13.11±0.19)mg/g.The antioxidant test results showed that the total antioxidant capacity and hydroxyl radical scavenging rate of coriander polyphenol extract increased with increasing concentration.The total antioxidant capacity of coriander polyphenol extract at a concentration of 0.6 mg/mL was(102±2)μmol/L,and the hydroxyl radical scavenging rate was(60±1)%.The antibacterial test results showed that coriander polyphenols had inhibitory effects on Escherichia coli and Staphylococcus aureus,and the antibacterial activity increased with increasing polyphenol concentration.The inhibition zone diameter of the coriander polyphenol extract at a concentration of 0.6 mg/mL against E.coli was(6.76±0.02)mm,and the inhibition zone diameter against S.aureus was(7.04±0.02)mm.
keywords: coriander polyphenols ultrasonic-assisted extraction method total antioxidant capacity hydroxyl radical scavenging rate antibacterial activity
文章编号:202315016 中图分类号: 文献标志码:
基金项目:山西省重点研发计划项目(201903D221066);山西省基础研究计划青年项目(202203021212310);太原科技大学博士科研启动基金项目(20192046、20192051);山西省高等学校科技创新项目(2020L0364)
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