Abstract:In order to improve the antioxidation of coffee，chlorogenic acid was added to coffee in this study and the mouse-based antioxidant experiment was carried out.The mice were randomly divided into model group，VC group as well as high-，medium-and low-dose experimental groups.The oxidative stress model was established by intragastric administration of D-galactose.The body mass，blood routine，the content of malondialdehyde（MDA），the activity of superoxide dismutase（SOD），the activity of glutathione peroxidase（GSH-Px）in the liver and the immune organ indexes in mice were measured.The results showed that there was no significant difference in body mass and leukocyte number in blood routine between groups （p＞0.05）.The medium-dose chlorogenic acid group showed no significant difference （p＞0.05）with the blank group and the positive control group in the content of mean corpuscular hemoglobin（MCH），the number of granulocytes（GRA），thymus index，and spleen index，in the middle dose group of chlorogenic acid but had significant differences with the model control group and the coffee control group （p＜0.05）.Compared with those in the model control group，the MCH content，GRA number and liver MDA content in the medium-dose chlorogenic acid group were significantly decreased（p＜0.05），and the SOD activity，GSH-Px activity，thymus index and spleen index were significantly increased（p＜0.05），which proved that the coffee added with chlorogenic acid could ameliorate the oxidative stress damage induced by D-galactose in mice to a certain degree.