Abstract:In order to better assist and study Ganoderma lucidum polysaccharides and further improve the antioxidant activity of Ganoderma lucidum polysaccharides in cell wall matrix of spore subphylum entity（Ganoderma lucidum fruit body），Candida utilis and Saccharomycopsis fibuligera were selected as the mixed polysaccharide fermentation strains of Ganoderma lucidum.The response value was the free radical scavenging rate of DPPH from polysaccharide in the fruiting body of G.lucidum.Further，combined with a single-factor test，Box-Behnken response design response surface method was used to optimize the fermentation technology of polysaccharides in the fruiting body of G.lucidum.The results showed that the optimal liquid fermentation reaction conditions：the fermentation time was 60 h，the inoculated bacteria amount was 3%，pH4，and the fermentation temperature 26 ℃.Under the optimal fermentation conditions，the scavenging rate of DPPH radical was 90%.Shows that the polysaccharides from the fruiting body of G.lucidum after fermentation processing，it has quite strong natural antioxidant activity.