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投稿时间:2022-03-03
投稿时间:2022-03-03
中文摘要: 该文对不同处理方式得到的两种凉粉草多糖(Mesona chinensis polysaccharides,MCP)的理化性质和体外抗氧化活性进行研究,采用同步热分析仪、紫外光谱、红外光谱、X射线衍射、扫描电镜(scanning electron microscope,SEM)等方法表征多糖结构,从DPPH自由基清除率、ABTS+自由基清除率以及还原力方面考察多糖抗氧化活性。结果表明,在动态旋转水合半径上MCP-A溶液比MCP-B溶液黏度大;热分析结果表明,MCP-A比MCP-B具有更强的耐热性;红外光谱结果表明,两种多糖都具有多糖的特征吸收峰,MCP-A为α-糖苷键和β-糖苷键的吡喃型糖苷环骨架,MCP-B为β-糖苷键的吡喃型糖苷环骨架;X射线衍射图谱表明两种多糖主要形态以非晶体存在;SEM结果表明MCP-B比MCP-A具有更大比表面积。体外抗氧化结果显示,MCP-A清除DPPH自由基、ABTS+自由基的能力比MCP-B强,二者均有较强的还原力。研究表明,两种处理方法得多糖的理化性质和抗氧化存在明显差异,MCP-A具有更高的热稳定性和抗氧化能力,可作为可潜在的抗氧化食品成分用于食品加工。
Abstract:Mesona chinensis polysaccharides MCP-A and MCP-B were obtained by different treatments,and the physicochemical properties and in vitro antioxidant activities of the two polysaccharides were studied.The structure of polysaccharides was characterized by synchronous thermal analyzer,ultraviolet ray,infrared ray,X-ray diffraction,scanning electron microscope,etc.The antioxidant activities of polysaccharides were investigated from the aspects of DPPH,ABTS+free radical scavenging rate and reducing power.The results showed that MCP-A solution was more viscous than MCP-B solution on the dynamic rotational hydration radius.MCP-A had stronger heat resistance than MCP-B.Both of the two polysaccharides had absorption peaks with polysaccharide characteristics,MCP-A was the pyranoside ring skeleton of α-glycosidic bond and β-glycosidic bond,and MCP-B was the pyranoside ring skeleton of β-glycosidic bond.The X-ray diffraction pattern showed that the main forms of the two polysaccharides were amorphous.The scanning electron microscopy(SEM)showed that MCP-B had a larger specific surface area than MCP-A.The in vitro antioxidant results showed that MCP-A was stronger than MCP-B in scavenging DPPH and ABTS+free radicals,and both had stronger reducing power.The study indicated that the physicochemical properties and antioxidant activities of polysaccharides treated by two methods are significantly different.Compared with MCP-B,MCP-A had higher thermal stability and antioxidant capacity,which can be used as a potential antioxidant ingredient in food.
keywords: Mesona chinensis Benth polysaccharide alkali formulation physicochemical properties antioxidant activity
文章编号:202309008 中图分类号: 文献标志码:
基金项目:广东省科技计划项目(2020A1414010188);湛江市科技计划项目(2020A03027);粤西特色生物医药工程技术研究中心开放课题(2022-K12)
作者 | 单位 |
董伟1,2,马生健1*,马文欣1,罗艺婷1,吴彩艳1,李佳悦1,陆静恩1,杨桂容1 | 1.新疆农业大学机电工程学院,新疆乌鲁木齐 830052;2.岭南师范学院生命科学与技术学院,广东湛江 524048 |
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