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投稿时间：2022-03-21
投稿时间：2022-03-21
中文摘要: 采用单因素结合响应面法优化黑果腺肋花楸全果、果渣原花青素的提取工艺，并以DPPH自由基、ABTS+自由基、羟自由基清除率和总还原能力评估其抗氧化能力。结果表明，黑果腺肋花楸全果和果渣原花青素最佳提取工艺条件为液料比45∶1（mL/g），乙醇体积分数50%，提取时间37 min，提取温度50℃，在此工艺条件下花楸全果和果渣原花青素得率分别为98.48、78.04 mg/g。体外抗氧化研究结果显示，黑果腺肋花楸全果、果渣原花青素具有良好的还原能力且对 ABTS+自由基（IC50779.51、315.95 μg/mL）、DPPH 自由基（IC50406.73、156.73 μg/mL）和羟自由基（IC501 319.02、393.66 μg/mL）均具有一定的清除能力，其中对DPPH自由基清除能力最优。综上，黑果腺肋花楸果渣中仍含有大量原花青素，且抗氧化能力优于全果原花青素，具有进一步开发的潜力和良好的再利用价值。
Abstract:The single factor test was combined with response surface methodology to optimize the ultrasound-assisted extraction of proanthocyanidins from Aronia melanocarpa fruits and the marc.Their antioxidant activity was evaluated based on the ABTS+，DPPH，and hydroxyl radicals scavenging capacity and total reducing ability.The results showed that the optimal extraction conditions were as follows：liquid-to-material ratio of 45∶1（mL/g），50%ethanol，extraction for 37 min，and 50 ℃.At this optimal conditions，the yield of proanthocyanidins from the fruits and marc was 98.48 mg/g and 78.04 mg/g，respectively.The experiment on the in vitro antioxidant activity suggested that the proanthocyanidins from the fruits and marc had strong reducing ability and can scavenge ABTS+（IC50of 779.51 and 315.95 μg/mL），DPPH（IC50of 406.73 and 156.73 μg/mL），and hydroxyl radicals（IC50of 1 319.02 and 393.66 μg/mL），particularly DPPH radicals.In summary，the marc of A.melanocarpa contains a lot of proanthocyanidins with the antioxidant capacity stronger than that from the fruit，which should be further developed and utilized.
文章编号：202308014 中图分类号： 文献标志码：
基金项目:天津市科技计划项目（19YFZCSN00010）
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