Abstract:Filamentous fungi with both medicinal and edible values are important microbial resources in China and can produce rich beneficial primary and secondary metabolites.Their metabolism is regulated by a variety of regulatory factors.Velvet protein family is unique and highly conserved in fungi，which can regulate the growth，differentiation，and secondary metabolite biosynthesis of fungi. The structures of the Velvet protein family members Velvet A（VeA）and Velvet like B（VelB）in fungi were introduced，and the research progress in the regulatory effects of the VeA and VelB complexes on the growth，development，and secondary metabolism was reviewed.The review could provide reference for further exploring the regulatory role of Velvet protein family in the fungal synthesis of secondary metabolites，activating silenced gene clusters，and mining new compounds.