Abstract:In this study，eight different extraction methods were used for the extraction of polyphenols from hempseed threshing residues. By comparing the extraction rates and the in vitro antioxidant activities of polyphenols obtained via different extraction methods，the optimal extraction technology was selected. The macroporous resin was used to purify polyphenol crude extract samples，and the purification conditions were determined. Based on the results，ultrasonic alcohol extraction yielded the highest polyphenol extraction rate of 2.34 mg/g. The polyphenol extracts obtained via different extraction methods could scavenge DPPH·and ABTS+·，and the extract obtained via ultrasonic alcohol extraction showed strong scavenging ability at different concentration levels. The most suitable resin for purifying polyphenols from hempseed threshing residues was X-5，with adsorption and resolution rates of 74.5% and 44.5%，respectively. The concentration of the ethanol solution was 90%.The scavenging capacity of purified polyphenols on DPPH·and ABTS+·and the concentration of samples had a dose-response relationship，with IC50 values of 0.38 mg/mL and 0.10 mg/mL，respectively.The purified hempseed threshing polyphenols had high antioxidant activity levels.