Abstract:The enzyme extraction conditions of polysaccharides from the head of Perccottus glenii were optimized，and the antioxidant activity of the extracted polysaccharides in vitro was determined.On the basis of single factor tests，response surface methodology was employed to optimize the extraction process of polysaccharides from P.glenii.The antioxidant activity of polysaccharides in vitro was evaluated based on the hydroxyl and DPPH free radical scavenging rates，and the structure of polysaccharides was determined by Fourier transform infrared spectroscopy（FTIR）.The extraction conditions were optimized as pH6.5，enzyme dosage of 2.5%，solid-to-liquid ratio of 1∶30（g/mL），and enzymolysis at 37 ℃for 2 h，under which the yield of polysaccharides was 11.43%.The in vitro antioxidant test showed that the hydroxyl and DPPH free radical scavenging rates of 10 mg/mL polysaccharides were 81.2% and 80%，respectively. The FTIR result showed that the polysaccharides from the head of P.glenii might contain β-glycoside configuration and pyranoid ring. The findings of this study preliminarily revealed the properties of the polysaccharides from the head of P. glenii，providing a theoretical basis for the value-added application of by-products in aquatic product processing.