本文已被:浏览 598次 下载 436次
投稿时间:2021-03-04
投稿时间:2021-03-04
中文摘要: 为深入了解电子束辐照对猪肉风味的影响,对低剂量电子束辐照(1、2、3、4 kGy)处理的冷鲜猪里脊肉风味进行研究分析。采用快速气相色谱电子鼻、电子舌、顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)结合气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)技术以及邻苯二甲醛与氯甲酸芴甲酯联合柱前衍生法测定游离氨基酸,对其风味物质进行定性定量分析。游离氨基酸分析结果表明,辐照处理后1、2 kGy猪里脊肉中游离氨基酸总含量与对照组相比显著降低(P<0.05);电子舌从味感上分析发现辐照样品与对照组相比,在酸味、甜味、苦味和苦味回味上有差异;电子鼻结果显示辐照样品与对照组在嗅感上存在差异,其中2 kGy样品与未辐照样品挥发性风味物质最接近;同时,固相微萃取-气相色谱-质谱联用检测分析结果显示,辐照后样品中挥发性风味物质增加。研究发现电子束辐照对冷鲜猪里脊肉的风味有影响,不同辐照剂量对冷鲜猪里脊肉风味的影响程度也不同。
Abstract:In order to reveal the effect of electron beam irradiation on pork flavor,this study analyzed the flavor of chilled pork tenderloin treated by low-dose electron beam irradiation(1,2,3 kGy and 4 kGy).Using ultrafastgas chromatography(GC)coupled to electronic nose,electronic tongue,and headspace solid-phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS),as well as pre-column derivatization of phthalaldehyde and fluorenylmethyl chloroformate,the free amino acids were determined and the flavor substances were analyzed qualitatively and quantitatively.The results showed that the total content of free amino acids in the pork tenderloin after irradiation at 1 kGy and 2 kGy was lower than that in the control group(P<0.05).The electronic tongue results indicated that the irradiated samples were different from the control in sour taste,sweet taste,bitter taste,and bitter aftertaste.The electronic nose results showed that there were differences between the irradiated samples and the control in smell,and the sample irradiated at 2 kGy was the closest to the unirradiated sample in terms of volatile flavor substances.Meanwhile,the results of HSSPME-GC-MS showed that the volatile flavor substances in the irradiated samples increased.The findings of this study indicated that electron beam irradiation had an effect on the flavor of chilled pork tenderloin,and different irradiation doses had different effects on the flavor of chilled pork tenderloin.
keywords: electron beam irradiation pork tenderloin flavor electronic nose electronic tongue headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry
文章编号:202303011 中图分类号: 文献标志码:
基金项目:上海市科技兴农重点攻关项目(沪农科创字[2018]第1-6号)
作者 | 单位 |
石梦琦1,冯涛2,孔秋莲3,戚文元4,王亮1*,宋诗清2,姚凌云2,孙敏2,王化田2 | 1.新疆大学生命科学与技术学院,新疆 乌鲁木齐 830046;2.上海应用技术大学香料香精技术与工程学院,上海 201418;3.上海束能辐照技术有限公司,上海 201403;4.上海市农业科学院作物研究所,上海 201403 |
引用文本: