Abstract:The purpose of this study was to explore the anti-oxidant and anti-fatigue effects of Polygonatumbased wine on ICR male mice.The active components of Polygonatum plants were extracted by ethanol extraction，and edible ethanol was added to prepare Polygonatum-based wine.Sixty ICR male infant mice were randomly divided into the following six groups for anti-oxidant activity experiments:a blank group，a positive group（ascorbic acid，120 mg/kg），a negative group （white wine），and high-，medium-，and low-dose groups of Polygonatum-based wine（33.4，16.7，and 8.4 mL/kg）.A total of 180 mice were assigned to the following groups for the anti-fatigue experiments:a blank group，a control group（salidroside，120 mg/kg），a negative group（white wine），and high-，medium-，and low-dose groups of Polygonatum-based wine（33.4，16.7，and 8.4 mL/kg）.The anti-oxidant effect of Polygonatum-based wine on healthy mice was assessed by measuring the weight of mice in each group，the content of malondialdehyde and glutathione in the liver and kidney tissues，and the activities of catalase and superoxide dismutase.The anti-fatigue effect of Polygonatum-based wine on mice was assessed by recording the weight-bearing swimming time and the content of liver glycogen，whole blood lactic acid，and the activities of alanine aminotransferase of the mice in each group.The results showed that Polygonatum-based wine could effectively reduce the content of malondialdehyde and glutathione in the body，enhance the activities of superoxide dismutase，catalase（P<0.05），prolong the weight-bearing swimming time（P<0.05），elevate the liver glycogen content，decrease blood lactic acid content，and blunt alanine aminotransferase activity（P<0.05）.In conclusion，Polygonatum-based wine had the effects of relieving fatigue and improving the antioxidant capacity of mice.