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投稿时间:2021-12-29
投稿时间:2021-12-29
中文摘要: 为确定脆红李果实采后主要病原菌的种类及茉莉酸甲酯对其抑制效果,从脆红李贮藏期病果中分离纯化病原菌,通过致病性检测、形态学鉴定和rDNA-ITS序列分析对病原菌进行鉴定;并采用体外实验研究不同浓度的茉莉酸甲酯(0、0.1、0.2、0.3、0.4、0.5 mmol/L)对脆红李采后主要致病菌的抑制效果及室内毒力测定。结果表明,引起脆红李采后腐烂的病原菌主要为灰葡萄孢菌(Botrytis cinerea)和链格孢菌(Alternaria alternata);经体外实验得出,不同浓度的茉莉酸甲酯能抑制两株病原菌菌丝的生长,其中,0.4 mmol/L及0.5 mmol/L的茉莉酸甲酯能够有效抑制脆红李采后病原菌的生长,但其对病原菌的抑制作用无显著差异(P>0.05);在体外培养5 d时,0.4 mmol/L的茉莉酸甲酯对灰葡萄孢菌和链格孢菌的抑制率分别为89.3%、86.3%;在培养2 d时,茉莉酸甲酯对灰葡萄孢菌和链格孢菌的EC50分别为0.154、0.228 mmol/L,而在5 d时为0.193、0.348 mmol/L。由此得出适宜浓度的茉莉酸甲酯能够有效抑制脆红李贮藏期病原菌的生长,后期可将其用于脆红李采后病害的防治及贮藏保鲜的研究。
Abstract:To determine the main pathogens of Prunus salicina after harvest and explore the inhibitory effect of methyl jasmonate on its postharvest storage disease,the pathogens were isolated and purified from the diseased fruit of P.salicina during storage.The pathogens were identified by pathogenicity detection,morphological identification,and rDNA-ITS sequence analysis.In vitro experiments were carried out to study the inhibitory effect of different concentrations of methyl jasmonate (0,0.1,0.2,0.3,0.4 mmol/L,and 0.5 mmol/L)on the main pathogens of P.salicina and measure the indoor virulence.The results showed that the main pathogens causing postharvest decay of P.salicina were Botrytis cinerea and Alternaria alternate.In vitro experiments showed that different concentrations of methyl jasmonate inhibited the mycelial growth of the two strains of pathogens,among which 0.4 mmol/L and 0.5 mmol/L methyl jasmonate effectively inhibited the growth of pathogens of P.salicina after harvest,but there had no significant difference in their inhibitory effects on the pathogens(P>0.05).The inhibition rates of 0.4 mmol/L methyl jasmonate on Botrytis cinerea and Alternaria alternata were 89.3%and 86.3%,respectively,on the 5thday of in vitro cultivation.The EC50of methyl jasmonate on Botrytis cinerea and Alternaria alternata were 0.154 mmol/L and 0.228 mmol/L,respectively,on the 2nd day of in vitro cultivation,and were 0.193 mmol/L and 0.348 mmol/L on the 5th day of in vitro cultivation.It is concluded that the appropriate concentration of methyl jasmonate can effectively inhibit the growth of pathogens in P.salicina during storage,which can be used in the postharvest disease control and the storage and preservation of P.salicina in the later stage.
keywords: Prunus salicina pathogens isolation and identification methyl jasmonate bacteriostatic effect
文章编号:202224014 中图分类号: 文献标志码:
基金项目:贵阳市科技计划项目(筑科合同[2020]37-1号);贵阳学院科研资金资助项目(GYU-KY-[2022])
Author Name | Affiliation |
QU Guang-fan,BA Liang-jie,LEI Qi-qing,MA Chao,ZHANG Xu-yang,CAO Sen* | Food and Pharmaceutical Engineering Institute,Guiyang University,Guiyang 550005,Guizhou,China |
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