Abstract:Miscanthus lutarioriparius was used as raw material to study the effects of formic acid（FA）combined with alkaline hydrogen peroxide （AHP）on the enzymatic hydrolysis characteristics of cellulose from M.lutarioriparius，and the physicochemical structures of the samples before and after treatment were characterized by Fourier transform infrared spectroscopy，X-ray diffraction spectroscopy，and scanning electron microscopy.The results showed that the cellulose content of M.lutarioriparius AHP cellulose obtained by FA combined with AHP was significantly increased to 89.85%，whereas the content of lignin and hemicellulose was significantly decreased to 3.49%and 3.72%，respectively.As compared with M.lutarioriparius FA cellulose，the formylation modification disappeared，the crystallinity index decreased，and more cellulose was exposed in M.lutarioriparius AHP cellulose，which greatly improved the transformation efficiency of enzymatic hydrolysis.The conversion rate of M.lutarioriparius AHP cellulose reached 95.54%after 120 h at the substrate concentration of 5%.When the substrate concentration was 20%，the glucose concentration in the enzymatic solution was up to 133.17 g/L，which was significantly higher than that of raw material and M.lutarioriparius FA cellulose.The results of this study provide technical support and a theoretical basis for the efficient conversion of M.lutarioriparius cellulose to clean energy.